Mapping of Myxococcus xanthus social motility dsp mutations to the dif genes

J Bacteriol. 2002 Mar;184(5):1462-5. doi: 10.1128/JB.184.5.1462-1465.2002.

Abstract

Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes. Another dsp allele contains a mutation in the sglK gene. About 36.6 kb around the dsp-dif locus was sequenced and annotated, and 50% of the genes are novel.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Chromosome Mapping*
  • Chromosomes, Bacterial / genetics
  • Gene Deletion*
  • Genes, Bacterial*
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Movement
  • Myxococcus xanthus / genetics
  • Myxococcus xanthus / physiology*
  • Sequence Analysis, DNA

Substances

  • Bacterial Proteins

Associated data

  • GENBANK/AF449411