Abstract
A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of blaTEM-87 in Escherichia coli decreased the host susceptibility to these drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aztreonam / pharmacology
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Cephalosporins / metabolism
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Escherichia coli / drug effects
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Escherichia coli / genetics
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Humans
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Italy / epidemiology
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Kinetics
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Molecular Sequence Data
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Monobactams / pharmacology
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Proteus Infections / epidemiology*
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Proteus Infections / microbiology*
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Proteus mirabilis / enzymology*
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Reverse Transcriptase Polymerase Chain Reaction
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beta-Lactamases / analysis
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beta-Lactamases / isolation & purification
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beta-Lactamases / metabolism*
Substances
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Cephalosporins
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Monobactams
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beta-lactamase TEM-87
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beta-Lactamases
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Aztreonam