Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA

J Am Chem Soc. 2002 Feb 27;124(8):1568-9. doi: 10.1021/ja017193q.

Abstract

Novel redox-active DNA labeling tags with tunable electrochemical potentials are modularly synthesized using (a) bis-substituted Ru2+ or Os2+ precursors (R2bpy)2ML2, (b) substituted 2,4-pentanediones or hydroxamic acids bearing a functionalized linker, and (c) modified nucleotides. DNA polymerase efficiently incorporates the metal-containing nucleotide triphosphate into DNA oligonucleotides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 2,2'-Dipyridyl / chemistry
  • DNA / chemistry*
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / metabolism
  • Electrochemistry
  • Nucleotides / chemical synthesis
  • Nucleotides / chemistry*
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Osmium / chemistry*
  • Oxidation-Reduction
  • Ruthenium / chemistry*
  • Thymine Nucleotides / chemical synthesis
  • Thymine Nucleotides / chemistry

Substances

  • Nucleotides
  • Organometallic Compounds
  • Thymine Nucleotides
  • Osmium
  • 2,2'-Dipyridyl
  • Ruthenium
  • DNA
  • DNA-Directed DNA Polymerase
  • thymidine 5'-triphosphate