Abstract
From the dried bulbs of the lily (Lilium brownii), a protein with strong antifungal and mitogenic activities was isolated. It also exhibited an inhibitory action on the activity of HIV-1 reverse transcriptase. The protein was single-chained and possessed a molecular weight of 14.4 kDa and an N-terminal sequence distinct from chitinases and antimicrobial proteins of garlic, leek and onion which belong to a family closely related to lily. However, there was a small degree of resemblance to cyclophilins and a considerable extent of identity to the 6.5 kDa arginine/glutamate-rich polypeptide from Luffa cylindrica seeds. A nearly homogeneous preparation was obtained after the extract was fractionated on DEAE-cellulose and Affi-gel Blue gel since subsequent chromatography on Mono S and Superdex 75 both yielded a single peak.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antifungal Agents / isolation & purification*
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Antifungal Agents / pharmacology
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Chromatography, Affinity
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Fungi / drug effects
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Fungi / growth & development
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HIV Reverse Transcriptase / antagonists & inhibitors
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Hemagglutination / drug effects
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Lilium / chemistry*
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Medicine, Chinese Traditional*
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Mice
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Mice, Inbred C57BL
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Microbial Sensitivity Tests
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Mitogens / isolation & purification*
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Molecular Sequence Data
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Plant Proteins / isolation & purification*
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Plant Proteins / pharmacology
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RNA, Plant / antagonists & inhibitors
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RNA, Plant / drug effects
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RNA, Transfer / drug effects
Substances
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Antifungal Agents
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Drugs, Chinese Herbal
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Mitogens
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Plant Proteins
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RNA, Plant
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lilin protein, Lilium brownii
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RNA, Transfer
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HIV Reverse Transcriptase