Sharpening spots: correcting for bleedover in cDNA array images

Math Biosci. 2002 Mar;176(1):1-15. doi: 10.1016/s0025-5564(01)00101-8.

Abstract

For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.

MeSH terms

  • Algorithms*
  • DNA, Complementary / analysis*
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / standards
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Multiple Myeloma / genetics
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oligonucleotide Array Sequence Analysis / standards

Substances

  • DNA, Complementary