Interferon-alpha resistance in renal carcinoma cells is associated with defective induction of signal transducer and activator of transcription 1 which can be restored by a supernatant of phorbol 12-myristate 13-acetate stimulated peripheral blood mononuclear cells

Br J Cancer. 2002 Feb 1;86(3):449-55. doi: 10.1038/sj.bjc.6600066.

Abstract

Therapy of selected human malignancies with interferon-alpha is widely accepted but often complicated by the emergence of interferon-alpha resistance. Interferon is a pleiotropic cytokine with antiproliferative, antitumour, antiviral and immunmodulatory effect; it signals through the Jak-STAT signal transduction pathway where signal transducer and activator of transcription 1 plays an important role. Here we report both, a lack of signal transducer and activator of transcription induction in interferon-alpha resistant renal cell carcinoma cells and signal transducer and activator of transcription 1 reinduction of phorbol 12-myristate 13-acetate-stimulated peripheral blood mononuclear cells supernatant. Preliminary experiments on the identification of the molecules that reinducing signal transducers and activators of transcription 1 indicate that interferon-gamma may be the responsible candidate cytokine, but several others may be involved as well. This work provides the basis for therapeutic strategies directed at the molecular modulation of interferon-alpha resistance in human neoplasms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Carcinoma, Renal Cell / physiopathology*
  • DNA-Binding Proteins / biosynthesis
  • Drug Resistance, Neoplasm / physiology*
  • Humans
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Kidney Neoplasms / physiopathology*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / physiology*
  • Recombinant Proteins
  • STAT1 Transcription Factor
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Trans-Activators / biosynthesis
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • DNA-Binding Proteins
  • Interferon alpha-2
  • Interferon-alpha
  • Recombinant Proteins
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • Trans-Activators
  • Tetradecanoylphorbol Acetate