Regulation of calcium currents by chemokines and their receptors

J Neuroimmunol. 2002 Feb;123(1-2):66-75. doi: 10.1016/s0165-5728(01)00485-4.

Abstract

We investigated the modulation of voltage dependent Ca(2+) currents by chemokine receptors in heterologous expression systems and neurons. Fractalkine, SDF-1alpha, RANTES and MDC inhibited the I(Ba) in CX3CR1-, CXCR4-, CCR5- and CCR4-expressing G1A1 cells, respectively. The I(Ba) inhibition was voltage-dependent, exhibited prepulse facilitation, and was blocked by N-ethylmaleimide and pertussis toxin pretreatment, indicating that it was mediated by Gi/Go. Some chemokines also inhibited the I(Ba) in subpopulations of dorsal root ganglion neurons and area postrema/nucleus tractus solitarius neurons. These data provide evidence that chemokines can potentially modulate neuronal signaling through the inhibition of neuronal Ca(2+) currents.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium Channels / drug effects*
  • Cell Line
  • Chemokines / pharmacology*
  • Female
  • GTP-Binding Protein alpha Subunits, Gi-Go / physiology
  • Heterotrimeric GTP-Binding Proteins / physiology
  • Neurons / metabolism*
  • Rats
  • Receptors, Cell Surface / physiology
  • Receptors, Chemokine / physiology*

Substances

  • Calcium Channels
  • Chemokines
  • Receptors, Cell Surface
  • Receptors, Chemokine
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Heterotrimeric GTP-Binding Proteins