Abstract
We recently found that human CD83, a marker of mature dendritic cells, is an adhesion receptor that binds to resting monocytes and a subset of activated CD8(+) T cells. We injected CD83-Ig into mice transplanted with the immunogenic P815 mastocytoma and showed that it significantly enhanced the rate of tumor growth and inhibited the development of cytotoxic T cells. In contrast, mice immunized with CD83-transfected K1735 cells, a poorly immunogenic melanoma, could prevent the outgrowth of wild-type K1735 cells. Studies performed in vitro with human PBL showed that coimmobilized CD83-Ig and anti-CD3 enhanced T cell proliferation and increased the proportion of CD8(+) T cells. CD83-transfected B-lymphoblastoid T51 cells stimulated T cell proliferation more effectively than untransfected T51 cells in MLR cultures and increased the generation of cytolytic T cells. We conclude that CD83 is a functionally important receptor that can regulate the development of cellular immunity by interacting with its ligand(s).
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / metabolism
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Antigens, CD
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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CD3 Complex / immunology
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CD8-Positive T-Lymphocytes / immunology
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CD83 Antigen
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Cell Division / genetics
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Cell Division / immunology
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Cell Line, Transformed
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Cytotoxicity, Immunologic / genetics
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Female
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Humans
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Immunity, Cellular / genetics
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Immunity, Cellular / immunology*
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Immunoglobulins / administration & dosage
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Immunoglobulins / genetics
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Immunoglobulins / metabolism
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Immunoglobulins / physiology*
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Immunosuppressive Agents / pharmacology
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Injections, Intraperitoneal
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K562 Cells
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Lymphocyte Activation / genetics
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Lymphocyte Activation / immunology
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Lymphocyte Culture Test, Mixed
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Membrane Glycoproteins / administration & dosage
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Membrane Glycoproteins / physiology*
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Mice
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Mice, Inbred C3H
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Mice, Inbred DBA
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Neoplasm Transplantation
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Recombinant Fusion Proteins / administration & dosage
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Recombinant Fusion Proteins / genetics
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Solubility
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T-Lymphocytes / immunology
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD3 Complex
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Immunoglobulins
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Immunosuppressive Agents
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Membrane Glycoproteins
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Recombinant Fusion Proteins