Genetic susceptibility of term pregnant women to oxidative damage

Toxicol Lett. 2002 Mar 28;129(3):255-62. doi: 10.1016/s0378-4274(02)00014-0.

Abstract

Genetic polymorphisms involved in the activation and detoxification of exogenous chemicals and in the production and scavenging of reactive oxygen species may modulate the levels of oxidative injury biomarker. We investigated 81 pregnant women in Inchon, Korea. In addition to a questionnaire survey, urinary concentrations of 8-hydroxydeoxyguanosine (8-OH-dG) and malondialdehyde (MDA) were measured as oxidative injury biomarkers. Cytochrome P-450(CYP)1A1, CYP2E1, glutathione S-transferase (GST)M1 and GSTT1 polymorphisms and myeloperoxidase (MPO) and manganese superoxide dismutase (MnSOD) polymorphisms were evaluated to determine the effect of genetic modification on urinary 8-OH-dG and MDA. The concentrations of urinary 8-OH-dG were significantly elevated in the presence of the MnSOD variant genotype (P=0.04) and in the case of GSTM1 null status (P=0.02) by multivariate regression. The concentrations of urinary MDA were not affected significantly by the genetic polymorphisms. This result shows that oxidative stress injury is modified by some heritable polymorphisms, including GSTM1 and MnSOD.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Biomarkers / urine
  • Cytochrome P-450 CYP1A1 / genetics
  • Cytochrome P-450 CYP2E1 / genetics
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Female
  • Genetic Predisposition to Disease*
  • Genotype
  • Glutathione Transferase / genetics
  • Humans
  • Malondialdehyde / urine
  • Oxidative Stress / genetics*
  • Peroxidase / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Polymorphism, Restriction Fragment Length
  • Pregnancy
  • Superoxide Dismutase / genetics
  • Surveys and Questionnaires

Substances

  • Biomarkers
  • Malondialdehyde
  • 8-Hydroxy-2'-Deoxyguanosine
  • Peroxidase
  • Cytochrome P-450 CYP2E1
  • Cytochrome P-450 CYP1A1
  • Superoxide Dismutase
  • glutathione S-transferase T1
  • Glutathione Transferase
  • glutathione S-transferase M1
  • Deoxyguanosine