Abstract
Antibiotic susceptibility testing by disk diffusion of a Chryseobacterium gleum isolate, strain CIP 103039, showed a typical synergy image between clavulanic acid and expanded-spectrum cephalosporins. Shotgun cloning gave a recombinant plasmid in Escherichia coli that produced a beta-lactamase, CGA-1, with a pI value of 8.9 that conferred resistance to most penicillins (except ureidopenicillins) and narrow-spectrum cephalosporins and an intermediate susceptibility to expanded-spectrum cephalosporins and aztreonam. The CGA-1 amino acid sequence shared only 60% amino acid identity with CME-1 and CME-2 from Chryseobacterium meningosepticum, the most closely related beta-lactamases. CGA-1 was very likely chromosome encoded. It is a novel member of the PER subgroup of Ambler class A beta-lactamases (Bush functional group 2be).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacteria / drug effects
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Bacteria / enzymology*
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Bacteria / genetics
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Bacterial Proteins / chemistry
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Ceftazidime / pharmacology
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Cephalosporins / pharmacology
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Clavulanic Acid / pharmacology
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Conjugation, Genetic / genetics
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Enzyme Induction / drug effects
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Isoelectric Focusing
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Kinetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Molecular Weight
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Plasmids / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Cephalosporins
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Recombinant Proteins
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Clavulanic Acid
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Ceftazidime
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CGA-1 beta-lactamase
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beta-Lactamases