Development of an RT-PCR on the heat shock protein 70 gene for viability detection of Pneumocystis carinii f. sp. hominis in patients with pneumocystosis and in air sample

S Latouch; A Totet; P Lacube; J Bolognini; G Nevez; P Roux

doi:10.1111/j.1550-7408.2001.tb00508.x

Development of an RT-PCR on the heat shock protein 70 gene for viability detection of Pneumocystis carinii f. sp. hominis in patients with pneumocystosis and in air sample

J Eukaryot Microbiol. 2001:Suppl:176S-177S. doi: 10.1111/j.1550-7408.2001.tb00508.x.

Authors

S Latouch¹, A Totet, P Lacube, J Bolognini, G Nevez, P Roux

Affiliation

¹ Laboratoire de Parasitologie-Mycologie, CHU Saint-Antoine, Paris, France. [email protected]

PMID: 11906051
DOI: 10.1111/j.1550-7408.2001.tb00508.x

Abstract

To test the viability of Pneumocystis carinii f. sp. hominis, an RT-PCR assay that employs specific primers from the Heat Shock Protein 70 gene was developed. Using this method, the viability of P.c. hominis in bronchoalveolar lavage fluids from patients developing PCP and in the environment of PCP patients was established.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

AIDS-Related Opportunistic Infections / microbiology
Air Microbiology*
Air Pollution, Indoor
Bronchoalveolar Lavage Fluid / microbiology*
DNA, Fungal / analysis
HSP70 Heat-Shock Proteins / genetics*
Humans
Pneumocystis / genetics
Pneumocystis / growth & development*
Pneumocystis / isolation & purification
Pneumonia, Pneumocystis / microbiology*
RNA, Fungal / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction*

Substances

DNA, Fungal
HSP70 Heat-Shock Proteins
RNA, Fungal