Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis

Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):706-8. doi: 10.1107/s0907444902002640. Epub 2002 Mar 22.

Abstract

Selenate reductase from Thauera selenatis was crystallized using ammonium sulfate as a precipitant. Crystals of selenate reductase belong to the space group C2, with unit-cell parameters a = 116.9, b = 67.5, c = 186.7 A, beta = 90 degrees. Native data to 2.1 A resolution have been collected and a heavy-atom derivative has been identified following soaking of the crystals in a solution of trimethyl lead acetate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Oxidoreductases / chemistry*
  • Thauera / chemistry*

Substances

  • Oxidoreductases
  • selenate reductase