Nuclear receptor function requires a TFTC-type histone acetyl transferase complex

Junn Yanagisawa; Hirochika Kitagawa; Mitsuaki Yanagida; Osamu Wada; Satoko Ogawa; Madoka Nakagomi; Hajime Oishi; Yasuji Yamamoto; Hiromich Nagasawa; Steven B McMahon; Michael D Cole; Laszlo Tora; Nobuhiro Takahashi; Shigeaki Kato

doi:10.1016/s1097-2765(02)00478-1

Nuclear receptor function requires a TFTC-type histone acetyl transferase complex

Mol Cell. 2002 Mar;9(3):553-62. doi: 10.1016/s1097-2765(02)00478-1.

Authors

Junn Yanagisawa¹, Hirochika Kitagawa, Mitsuaki Yanagida, Osamu Wada, Satoko Ogawa, Madoka Nakagomi, Hajime Oishi, Yasuji Yamamoto, Hiromich Nagasawa, Steven B McMahon, Michael D Cole, Laszlo Tora, Nobuhiro Takahashi, Shigeaki Kato

Affiliation

¹ Institute of Molecular and Cellular Biosciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.

PMID: 11931763
DOI: 10.1016/s1097-2765(02)00478-1

Abstract

Nuclear receptors (NRs) regulate transcription in a ligand-dependent way through two types of coactivator complexes: the p160/CBP histone acetyl transferase (HAT) complex and the DRIP/TRAP/SMCC complex without HAT activity. Here we identified a large human (h) coactivator complex necessary for the estrogen receptor alpha (ERalpha) transactivation. This complex contains the GCN5 HAT, the c-Myc interacting protein TRRAP/PAF400, TAF(II)30, and other subunits. Similarly to known TFTC (TBP-free TAF(II)-containing)-type HAT complexes (hTFTC, hPCAF, and hSTAGA), TRRP directly interacted with liganded ER alpha, or other NRs. ER alpha transactivation was enhanced by the purified complex in vitro. Antisense TRRAP RNA inhibited estrogen-dependent cell growth of breast cancer cells. Thus, the isolated TFTC-type HAT complex acts as a third class of coactivator complex for NR function.

Publication types

Research Support, Non-U.S. Gov't
Retracted Publication

MeSH terms

Acetyltransferases / genetics
Acetyltransferases / metabolism*
Adaptor Proteins, Signal Transducing
Amino Acid Motifs
Cell Cycle Proteins
Cell Line
Cell Nucleus / chemistry
Cell Nucleus / metabolism
Estrogen Receptor alpha
Histone Acetyltransferases
Humans
Ligands
Macromolecular Substances
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Protein Binding
RNA, Antisense / genetics
RNA, Antisense / metabolism
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism
Receptors, Estrogen / genetics
Receptors, Estrogen / metabolism*
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae Proteins*
Trans-Activators / genetics
Trans-Activators / metabolism
Transcription Factors
Transcriptional Activation / genetics*
p300-CBP Transcription Factors

Substances

Adaptor Proteins, Signal Transducing
Cell Cycle Proteins
Estrogen Receptor alpha
Ligands
Macromolecular Substances
Nuclear Proteins
RNA, Antisense
Receptors, Cytoplasmic and Nuclear
Receptors, Estrogen
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Trans-Activators
Transcription Factors
transformation-transcription domain-associated protein
Acetyltransferases
Histone Acetyltransferases
KAT2A protein, human
KAT7 protein, human
p300-CBP Transcription Factors
p300-CBP-associated factor