Abstract
Androgen receptor (AR) is required for sexual differentiation and is implicated in the development of prostate cancer. Here we describe distinct functions for cofactor proteins and gene regulatory elements in the assembly of AR-mediated transcription complexes. The formation of an activation complex involves AR, coactivators, and RNA polymerase II recruitment to both the enhancer and promoter, whereas the formation of a repression complex involves factors bound only at the promoter and not the enhancer. These results suggest a model for the functional coordination between the promoter and enhancer in which communication between these elements is established through shared coactivators in the AR transcription complex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / metabolism
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Cell Cycle / physiology
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Cell Line
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DNA-Binding Proteins / metabolism
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Dihydrotestosterone / metabolism
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Enhancer Elements, Genetic*
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Gene Expression Regulation*
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Histone Acetyltransferases
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Humans
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Macromolecular Substances
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Models, Genetic
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Nuclear Receptor Coactivator 2
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Promoter Regions, Genetic*
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Receptors, Androgen / genetics*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription, Genetic*
Substances
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DNA-Binding Proteins
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Macromolecular Substances
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Nuclear Receptor Coactivator 2
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Receptors, Androgen
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Dihydrotestosterone
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Acetyltransferases
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Histone Acetyltransferases