Abstract
Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Biomarkers / analysis
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Cell Compartmentation / drug effects
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Cell Compartmentation / physiology
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cytoplasm / drug effects
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Cytoplasm / metabolism
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Cytoplasm / ultrastructure
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Extracellular Matrix Proteins / metabolism*
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Extracellular Matrix Proteins / pharmacology
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Fluorescent Antibody Technique
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Ganglia, Spinal / drug effects
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Ganglia, Spinal / embryology*
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Ganglia, Spinal / metabolism
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Humans
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Membrane Proteins / drug effects
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Membrane Proteins / metabolism*
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Models, Biological
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Nerve Tissue Proteins / drug effects
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Nerve Tissue Proteins / metabolism*
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Neural Crest / drug effects
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Neural Crest / embryology*
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Neural Crest / metabolism
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Neurites / drug effects
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Neurites / metabolism*
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Neurites / ultrastructure
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Neuroblastoma
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Neurofilament Proteins / drug effects
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Neurofilament Proteins / metabolism*
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Phosphorylation
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Rats
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Synaptosomal-Associated Protein 25
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Tumor Cells, Cultured
Substances
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Biomarkers
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Extracellular Matrix Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Neurofilament Proteins
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SNAP25 protein, human
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Snap25 protein, rat
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Synaptosomal-Associated Protein 25
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neurofilament protein H
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neurofilament protein M