Thyroid hormone increases transcription of GA-binding protein/nuclear respiratory factor-2 alpha-subunit in rat liver

Angeles Rodríguez-Peña; Héctor Escrivá; Ana C Handler; Carmen G Vallejo

doi:10.1016/s0014-5793(02)02389-x

Thyroid hormone increases transcription of GA-binding protein/nuclear respiratory factor-2 alpha-subunit in rat liver

FEBS Lett. 2002 Mar 13;514(2-3):309-14. doi: 10.1016/s0014-5793(02)02389-x.

Authors

Angeles Rodríguez-Peña¹, Héctor Escrivá, Ana C Handler, Carmen G Vallejo

Affiliation

¹ Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain.

PMID: 11943172
DOI: 10.1016/s0014-5793(02)02389-x

Abstract

Thyroid hormone (TH) regulates mitochondrial respiratory rate by activating coordinated transcription in the nucleus and mitochondria. Whereas TH activates transcription of mitochondrial genes directly, the activation of nuclear-encoded mitochondrial genes is probably executed by indirect unknown mechanisms. Nuclear respiratory factors (NRF)-1 and GA-binding protein (BP)/NRF-2 may function as transacting genes, but regulation of these genes by TH is not demonstrated. We show that TH administration to hypothyroid rats promptly increases GABP/NRF-2 alpha-subunit mRNA levels in the liver, without significant changes in beta, gamma subunits. In run-on and time-course experiments, the transcription rate and protein levels increased three-fold in response to TH, indicating GABP/NRF-2 transcriptional regulation. The results also support the notion that ATP synthase beta-subunit is regulated by TH through the indirect activation of GABP/NRF-2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ATP Synthetase Complexes / genetics
ATP Synthetase Complexes / metabolism
Animals
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Drug Administration Schedule
GA-Binding Protein Transcription Factor
Gene Expression Regulation / drug effects
Gene Expression Regulation / physiology
Hypothyroidism / drug therapy
Hypothyroidism / metabolism
Injections, Intraperitoneal
Liver / drug effects*
Liver / metabolism
Male
Mitochondrial Proteins*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Protein Subunits
RNA, Messenger / metabolism
Rats
Rats, Wistar
Time Factors
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcription, Genetic / drug effects*
Triiodothyronine / administration & dosage*

Substances

DNA-Binding Proteins
GA-Binding Protein Transcription Factor
Gabpa protein, rat
Gabpb2 protein, rat
Mitochondrial Proteins
Nuclear Proteins
Protein Subunits
RNA, Messenger
Tfam protein, rat
Transcription Factors
mitochondrial transcription factor A
Triiodothyronine
ATP Synthetase Complexes