Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex

Ryuichi Nishihama; Takashi Soyano; Masaki Ishikawa; Satoshi Araki; Hirokazu Tanaka; Tetsuhiro Asada; Kenji Irie; Mayumi Ito; Mizuya Terada; Hiroharu Banno; Yoshiko Yamazaki; Yasunori Machida

doi:10.1016/s0092-8674(02)00691-8

Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex

Cell. 2002 Apr 5;109(1):87-99. doi: 10.1016/s0092-8674(02)00691-8.

Authors

Ryuichi Nishihama¹, Takashi Soyano, Masaki Ishikawa, Satoshi Araki, Hirokazu Tanaka, Tetsuhiro Asada, Kenji Irie, Mayumi Ito, Mizuya Terada, Hiroharu Banno, Yoshiko Yamazaki, Yasunori Machida

Affiliation

¹ Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, 464-8602, Nagoya, Japan.

PMID: 11955449
DOI: 10.1016/s0092-8674(02)00691-8

Abstract

The tobacco mitogen-activated protein kinase kinase kinase NPK1 regulates lateral expansion of the cell plate at cytokinesis. Here, we show that the kinesin-like proteins NACK1 and NACK2 act as activators of NPK1. Biochemical analysis suggests that direct binding of NACK1 to NPK1 stimulates kinase activity. NACK1 is accumulated specifically in M phase and colocalized with NPK1 at the phragmoplast equator. Overexpression of a truncated NACK1 protein that lacks the motor domain disrupts NPK1 concentration at the phragmoplast equator and cell plate formation. Incomplete cytokinesis is also observed when expression of NACK1 and NACK2 is repressed by virus-induced gene silencing and in embryonic cells from Arabidopsis mutants in which a NACK1 ortholog is disrupted. Thus, we conclude that expansion of the cell plate requires NACK1/2 to regulate the activity and localization of NPK1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / embryology*
Arabidopsis / growth & development
Arabidopsis / metabolism
Base Sequence / genetics
Cell Division / physiology*
Cell Wall / metabolism*
Cell Wall / ultrastructure
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Gene Expression Regulation, Plant / physiology
Gene Silencing / physiology
Kinesins / genetics
Kinesins / metabolism
MAP Kinase Kinase Kinases / genetics
MAP Kinase Kinase Kinases / metabolism*
Molecular Motor Proteins / genetics*
Molecular Motor Proteins / isolation & purification
Molecular Sequence Data
Mutation / genetics
Nicotiana / embryology*
Nicotiana / growth & development
Nicotiana / metabolism
Phenotype
Phylogeny
Plant Proteins / genetics*
Plant Proteins / isolation & purification
Plant Proteins / metabolism*
Plant Structures / genetics
Plant Structures / metabolism
Plant Structures / ultrastructure
Protein Structure, Tertiary / genetics
Seeds / cytology
Seeds / embryology
Seeds / genetics
Sequence Homology, Amino Acid

Substances

DNA, Complementary
Molecular Motor Proteins
Plant Proteins
MAP Kinase Kinase Kinases
NPK1 protein, Nicotiana tabacum
Kinesins

Associated data

GENBANK/AB071435
GENBANK/AB071436
GENBANK/AB081599