Binding properties of oligonucleotides containing a modified 2'-deoxyuridine with a thymine ended linker to pair with 2'-deoxyadenosine

Bioorg Med Chem Lett. 2002 Mar 25;12(6):981-3. doi: 10.1016/s0960-894x(02)00062-8.

Abstract

Oligonucleotides containing in the place of thymidine the nucleoside 2, a 2'-deoxyuridine harbouring at C-5 a thymine ended linker, were found to undergo base pairing with the opposite 2'-deoxyadenosine. However, the corresponding duplexes are significantly destabilised as compared to the fully natural ones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cross-Linking Reagents / chemistry
  • Deoxyadenosines / chemistry*
  • Deoxyuridine / chemistry*
  • Drug Stability
  • Nucleic Acid Hybridization
  • Oligonucleotides / pharmacokinetics*
  • Thymine / chemistry

Substances

  • Cross-Linking Reagents
  • Deoxyadenosines
  • Oligonucleotides
  • 2'-deoxyadenosine
  • Thymine
  • Deoxyuridine