Abstract
This study was performed (i) to monitor the diffusion of the anti-cancer drug 5-fluorouracil (5-FU) and (ii) to elucidate the fate of poly(lactide-co-glycolide) (PLGA) based microspheres within living rat brain tissue upon intracranial implantation. Drug-loaded microparticles were prepared using a solvent emulsion/extraction process and administered into healthy and C6 glioma-bearing Sprague-Dawley rats. The same surgical procedure was carried out with magnetite-loaded microspheres. To monitor 5-FU diffusion from the implantation site, tissue combustion was performed on animals implanted with tritiated drug microspheres. T2-weighted nuclear magnetic resonance imaging was undertaken on animals implanted with magnetite-loaded microspheres to determine microsphere localization after deposit. Results show that an important microparticle backflow occurs in healthy rats, whereas the microspheres remain at the site of administration in C6 glioma-bearing rats. Drug diffusion is limited to the vicinity of the implantation site.
MeSH terms
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Animals
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Antineoplastic Agents / administration & dosage
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacokinetics*
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Biocompatible Materials / administration & dosage
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Biocompatible Materials / chemistry
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Biocompatible Materials / pharmacokinetics*
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Brain / metabolism*
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Brain Neoplasms / metabolism
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Contrast Media / pharmacokinetics
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Diffusion
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Drug Carriers / administration & dosage
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Drug Carriers / chemistry
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Drug Carriers / pharmacokinetics
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Female
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Ferrosoferric Oxide
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Fluorouracil / administration & dosage
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Fluorouracil / chemistry
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Fluorouracil / pharmacokinetics*
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Glioma / metabolism
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Injections
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Iron / pharmacokinetics
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Lactic Acid / administration & dosage
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Lactic Acid / chemistry
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Lactic Acid / pharmacokinetics*
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Microspheres
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Neoplasm Transplantation
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Oxides / pharmacokinetics
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Particle Size
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Polyglycolic Acid / administration & dosage
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Polyglycolic Acid / chemistry
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Polyglycolic Acid / pharmacokinetics*
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polymers / administration & dosage
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Polymers / chemistry
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Polymers / pharmacokinetics*
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Rats
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Rats, Sprague-Dawley
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Tritium
Substances
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Antineoplastic Agents
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Biocompatible Materials
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Contrast Media
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Drug Carriers
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Oxides
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Polymers
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Tritium
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Lactic Acid
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Iron
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Fluorouracil
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Ferrosoferric Oxide