Abstract
Occurrence of cnf1+ E. coli pathogenic strains among extraintestinal E. coli isolates was evaluated to explain an impact of cytotoxic necrotizing factor type 1 (CNF1) in human infections. A total of 120 E. coli isolates were characterized for presence of virulence factors cnf1- and pap--specific sequences by PCR, and the production of alpha-hemolysin using blood agar-plate test. Different association patterns among the detected virulence factors were obtained by comparison of various groups of clinical E. coli isolates. These differences probably reflect a potential impact of CNF1 in the colonization of vaginal environment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Bacterial Toxins / genetics*
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Bacterial Toxins / metabolism
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Child
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Child, Preschool
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Cytotoxins / genetics*
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Cytotoxins / metabolism
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Escherichia coli / genetics
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / microbiology*
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism
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Female
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Fimbriae, Bacterial / genetics
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Hemolysin Proteins / genetics*
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Hemolysin Proteins / metabolism
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Humans
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Infant
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Male
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Middle Aged
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Virulence / genetics
Substances
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Bacterial Toxins
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Cytotoxins
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Escherichia coli Proteins
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Hemolysin Proteins
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Hlya protein, E coli
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cytotoxic necrotizing factor type 1