Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis

J Clin Microbiol. 2002 May;40(5):1873-4. doi: 10.1128/JCM.40.5.1873-1874.2002.

Abstract

The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Colorimetry / economics
  • Colorimetry / methods
  • Costs and Cost Analysis
  • Microbial Sensitivity Tests / economics
  • Microbial Sensitivity Tests / methods
  • Mycobacterium tuberculosis / drug effects*
  • Peru
  • Reproducibility of Results

Substances

  • Anti-Bacterial Agents