Abstract
During the last few years, several genes, such as pap, bgl and flhDC, have been shown to be coregulated by the histone-like nucleoid-structuring (H-NS) protein and the cyclic AMP-catabolite activator protein (cAMP/CAP) complex, suggesting an interaction between both systems in the control of some cellular functions. In this study, the possible effect of H-NS on the cAMP level was investigated. In a CAP-deficient strain, the presence of an hns mutation results in a strong reduction in the amount of cAMP, due to a decrease in adenylate cyclase activity. This is caused by the reduced expression of crr, which encodes the Enzyme IIA(Glc) of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS), from its specific P2 promoter. This leads to a twofold reduction in the global amount of Enzyme IIA(Glc), the adenylate cyclase activator, responsible for the decrease in adenylate cyclase activity observed in the hns crp strain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenylyl Cyclases / biosynthesis
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Adenylyl Cyclases / genetics
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Adenylyl Cyclases / metabolism*
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Bacterial Proteins*
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Carrier Proteins
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Cyclic AMP / metabolism
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Cyclic AMP Receptor Protein / deficiency
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Cyclic AMP Receptor Protein / genetics
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DNA-Binding Proteins / deficiency
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DNA-Binding Proteins / genetics
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins
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Gene Expression Regulation, Bacterial
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Gene Expression Regulation, Enzymologic
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Mutation / genetics
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Operon / genetics
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
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Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism
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Phosphorylation
Substances
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Bacterial Proteins
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Carrier Proteins
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Cyclic AMP Receptor Protein
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DNA-Binding Proteins
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Escherichia coli Proteins
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H-NS protein, bacteria
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crr protein, E coli
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Cyclic AMP
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Phosphoenolpyruvate Sugar Phosphotransferase System
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Adenylyl Cyclases