Abstract
A novel transcript of human HPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated product of 23 kD different from the previous HPO that lacks the N-terminal 80 amino acids, was isolated from human fetal liver cDNA library by 5'RACE methods. The dose-dependent stimulation of DNA synthesis of HepG2 hepatoma cells by HPO-205 demonstrated its similar biological activity with HPO in vitro. The level of MAPK (mitogen-activated protein kinase)phosphorylation was further examined by Western blot analysis and the result revealed that HPO-205 protein might have stronger activity on stimulating hepatic cell proliferation than that of HPO. The similar result was observed by FACS technique. Therefore, our results suggest that the HPO-205 has activity to stimulate proliferation of liver derived cells, and this activity may be stronger than HPO.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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COS Cells
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Cell Cycle / drug effects
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Cell Division / drug effects
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Dose-Response Relationship, Drug
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Gene Expression
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Hepatocyte Growth Factor / genetics
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Hepatocyte Growth Factor / metabolism
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Hepatocyte Growth Factor / pharmacology*
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Humans
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Mitogen-Activated Protein Kinases / drug effects
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Mitogen-Activated Protein Kinases / metabolism
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Molecular Sequence Data
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Protein Isoforms / pharmacology
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RNA / genetics*
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RNA / isolation & purification
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RNA / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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Protein Isoforms
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RNA
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Hepatocyte Growth Factor
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Mitogen-Activated Protein Kinases