Abstract
Two strains (B728a and Y37) of the phytopathogenic bacterium Pseudomonas syringae pv. syringae isolated from bean (Phaseolus vulgaris) plants were shown to produce in culture both syringomycin, a lipodepsinonapeptide secreted by the majority of the strains of the bacterium, and a new form of syringopeptin, SP(22)Phv. The structure of the latter metabolite was elucidated by the combined use of mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy and chemical procedures. Comparative phytotoxic and antimicrobial assays showed that SP(22)Phv did not differ substantially from the previously characterized syringopeptin 22 (SP(22)) as far as toxicity to plants was concerned, but was less active in inhibiting the growth of the test fungi Rhodotorula pilimanae and Geotrichum candidum and of the Gram-positive bacterium Bacillus megaterium.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology
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Antifungal Agents / isolation & purification
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Antifungal Agents / pharmacology
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Bacterial Toxins / biosynthesis*
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Bacterial Toxins / isolation & purification
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Bacterial Toxins / pharmacology
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Chromatography, High Pressure Liquid
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Fabaceae / microbiology*
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Magnetic Resonance Spectroscopy / methods
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Mass Spectrometry
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Molecular Sequence Data
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Nicotiana / drug effects
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Peptides, Cyclic / biosynthesis*
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Peptides, Cyclic / isolation & purification
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Peptides, Cyclic / pharmacology
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Pseudomonas / chemistry
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Pseudomonas / metabolism*
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Species Specificity
Substances
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Anti-Bacterial Agents
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Antifungal Agents
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Bacterial Toxins
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Peptides, Cyclic
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syringopeptin SP(22)Phv