Abstract
The Jacob lectin, the most abundant glycoprotein in the cyst wall of Entamoeba invadens, contains five unique 6-Cys chitin-binding domains (CBDs). We identified Entamoeba histolytica and Entamoeba dispar genes encoding Jacob homologues, each of which contains two predicted 6-Cys CBDs. A unique 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lectins, called Jessie 1 to Jessie 3. The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2 and 3) were expressed in secretory vesicles of transfected amebae and shown to bind to particulate chitin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Chitin / metabolism*
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Chitinases / genetics*
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Chitinases / metabolism
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Cysteine / genetics*
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Cysteine / metabolism
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DNA, Protozoan
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Entamoeba histolytica / genetics*
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Entamoeba histolytica / metabolism
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Glycoproteins / genetics*
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Glycoproteins / metabolism
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Lectins / genetics*
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Lectins / metabolism
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Molecular Sequence Data
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Protozoan Proteins / genetics*
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Protozoan Proteins / metabolism
Substances
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DNA, Protozoan
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Glycoproteins
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Jacob protein, Entamoeba histolytica
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Lectins
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Protozoan Proteins
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Chitin
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Chitinases
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Cysteine
Associated data
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GENBANK/AF401984
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GENBANK/AF401985
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GENBANK/AF401986
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GENBANK/AF401988