The aim of this study was to determine the influence of skin sample site on the efficacy of trisodium phosphate (TSP) solutions in reducing Listeria monocytogenes populations on chicken carcasses during refrigerated storage. Chicken skin samples from the legs, the breasts, and the dorsal area inoculated with L. monocytogenes (10(8) CFU/ml) were dipped for 15 min in sterile tap water (control) or in 8, 10, or 12% TSP. L. monocytogenes counts and surface pH values were determined after 0, 1, 3, and 5 days of storage at 2 degrees C. For all sampling times and TSP concentrations, the reductions in L. monocytogenes numbers in breast skin were significantly larger (P < 0.05) than those in leg skin or dorsal skin. No significant differences were found in pH values as an effect of skin site. Our results suggest that skin sampling site is an important factor that needs to be considered when decontamination protocols are developed for poultry carcasses with the TSP treatment.