Abstract
We describe the design, synthesis and biophysical characterization of a novel DNA construct in which a folded quadruplex structure is joined to a standard double helix. Circular dichroism, gel electrophoresis, three-dimensional UV melting and differential scanning calorimetry were all used to characterize the structure. Rigorous molecular dynamics simulations were used to build a plausible atomic-level structural model of the DNA construct. This novel DNA construct provides a model for the duplex-quadruplex junction region at the end of chromosomal DNA and offers a system for the study of structure-selective ligand binding.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Calorimetry, Differential Scanning
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Circular Dichroism
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DNA / chemistry*
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DNA / genetics
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DNA / metabolism*
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DNA / radiation effects
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G-Quadruplexes
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Ligands
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Models, Molecular
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Nucleic Acid Conformation* / radiation effects
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Nucleic Acid Denaturation / radiation effects
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / genetics
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Oligodeoxyribonucleotides / metabolism
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Oligodeoxyribonucleotides / radiation effects
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Spectrometry, Fluorescence
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Telomere / chemistry*
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Telomere / genetics
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Telomere / metabolism*
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Telomere / radiation effects
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Thermodynamics
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Ultraviolet Rays
Substances
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Ligands
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Oligodeoxyribonucleotides
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DNA