Mammalian mitochondrial methylenetetrahydrofolate dehydrogenase-cyclohydrolase derived from a trifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase

Arch Biochem Biophys. 2002 Jul 1;403(1):145-8. doi: 10.1016/S0003-9861(02)00203-5.

Abstract

We have isolated the cDNA and the gene encoding the murine cytoplasmic methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (DCS). Comparison of these sequences with the 3'-untranslated region of the mitochondrial NAD(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase (mt-DC) revealed areas of significant homology. Both exon and intron sequences of the synthetase domain of DCS are homologous to sequences in the untranslated region of mt-DC. A similar comparison between the mt-DC and the DCS sequences of humans as well as Drosophila supports the conclusion that in higher eukaryotes the bifunctional mt-DC replaced a trifunctional precursor through inactivation of the synthetase domain. The mt-DC should be considered in models of one-carbon folate fluxes in mammals.

MeSH terms

  • 3' Untranslated Regions
  • Aminohydrolases / chemistry*
  • Aminohydrolases / genetics*
  • Animals
  • Base Sequence
  • Blotting, Southern
  • DNA, Complementary
  • Exons
  • Folic Acid / metabolism
  • Formate-Tetrahydrofolate Ligase / genetics*
  • Introns
  • Methylenetetrahydrofolate Dehydrogenase (NADP) / chemistry*
  • Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics*
  • Mice
  • Mitochondria / enzymology*
  • Models, Genetic
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry*
  • Multienzyme Complexes / genetics*
  • Protein Structure, Tertiary
  • Sequence Homology, Nucleic Acid

Substances

  • 3' Untranslated Regions
  • DNA, Complementary
  • Multienzyme Complexes
  • methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
  • formyl-methenyl-methylenetetrahydrofolate synthetase
  • Folic Acid
  • Methylenetetrahydrofolate Dehydrogenase (NADP)
  • Aminohydrolases
  • Formate-Tetrahydrofolate Ligase

Associated data

  • GENBANK/AF364580
  • GENBANK/AF364581
  • GENBANK/AF364582
  • GENBANK/AF364583
  • GENBANK/AF364584
  • GENBANK/AF364585
  • GENBANK/AF364586
  • GENBANK/AF364587
  • GENBANK/AF364588
  • GENBANK/AF364589
  • GENBANK/AF364590
  • GENBANK/AF364591
  • GENBANK/AF364592