Abstract
Based on the functional characterization of sucrose biosynthesis related protiens[SBP: sucrose-phosphate synthase (SPS), sucrose-phosphate phosphatase (SPP), and sucrose synthase (SuS)] in Anabaena sp. PCC7120 and sequence analysis, we have shown that SBP are restricted to cyanobacterium species and plants, and that they are multidomain proteins with modular architecture. Anabaena SPS, a minimal catalytic SPS unit, defines a glucosyltransferase domain present in all SPSs and SuSs. Similarly, Anabaena SPP defines a phosphohydrolase domain characteristic of all SPPs and some SPSs. Phylogenetic analysis points towards the evolution of modern cyanobacterial and plant SBP from a bidomainal common ancestral SPS-like gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anabaena / enzymology*
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Cyanobacteria / enzymology
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Evolution, Molecular
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Glucosyltransferases / chemistry*
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Glucosyltransferases / genetics
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Glucosyltransferases / metabolism*
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Molecular Sequence Data
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Phosphoprotein Phosphatases / chemistry*
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism
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Phosphoric Monoester Hydrolases / chemistry
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Phylogeny
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Plants / enzymology
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Sequence Alignment
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Sequence Analysis, Protein
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Sucrose / metabolism*
Substances
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Sucrose
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Glucosyltransferases
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sucrose synthase
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sucrose-phosphate synthase
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sucrose-phosphate synthase phosphatase
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Phosphoprotein Phosphatases
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Phosphoric Monoester Hydrolases
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sucrose-phosphatase