Detection of porcine circovirus 2 (PCV-2) DNA by nested PCR from formalin-fixed tissues of post-weaning multisystemic wasting syndrome (PMWS) pigs in Thailand

J Vet Med Sci. 2002 May;64(5):449-52. doi: 10.1292/jvms.64.449.

Abstract

A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circoviridae Infections / veterinary
  • Circoviridae Infections / virology*
  • Circovirus / genetics
  • Circovirus / isolation & purification*
  • DNA, Viral / analysis*
  • Polymerase Chain Reaction*
  • Swine / virology*
  • Swine Diseases / virology*
  • Wasting Syndrome / veterinary
  • Wasting Syndrome / virology*

Substances

  • DNA, Viral