Prevalence of superantigen-secreting bacteria in patients with Kawasaki disease

J Pediatr. 2002 Jun;140(6):742-6. doi: 10.1067/mpd.2002.123664.

Abstract

Objectives: To assess the prevalence of superantigen secreting bacteria in children with acute Kawasaki disease (KD) relative to control patients.

Study design: Bacterial cultures were obtained in a blinded fashion from the throat, rectum, and groin of 45 patients with untreated acute KD and 37 febrile control patients from 6 centers in the United States. Cultures were processed for the presence of superantigen-producing bacteria at a central laboratory.

Results: Staphylococci or streptococci that produced superantigens (TSST-1, SEB, SEC, SPEB, SPEC) were isolated from 25 of 45 patients with KD (56%) as compared with 13 of 37 (35%) control patients (P =.078). Because SEB- and SEC-producing Staphylococcus aureus have not been associated with KD and because they do not induce a Vbeta2+ T-lymphocyte response, we analyzed the difference between groups relative to superantigens TSST-1 or SPEB/SPEC production. TSST-1 secreting S aureus or SPEB/SPEC producing group A streptococci were isolated from 20 of 45 (44%) patients with KD compared with 7 of 37 (19%) control patients (P =.019).

Conclusions: The overall isolation rates of superantigen (TSST-1, SPEB, SPEC, SEB, SEC) producing bacteria between patients with KD and febrile control patients were not statistically significant. However, future studies should further examine the potential role of Vbeta2-stimulatory superantigens (TSST-1 and SPEB/SPEC) in KD.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Bacterial / isolation & purification*
  • Bacterial Proteins / immunology
  • Bacterial Toxins*
  • Calcium-Binding Proteins / immunology
  • Child
  • Child, Preschool
  • Cysteine Endopeptidases / immunology
  • Enterotoxins / immunology*
  • Enterotoxins / isolation & purification*
  • Female
  • Humans
  • Infant
  • Male
  • Mucocutaneous Lymph Node Syndrome / microbiology*
  • Prospective Studies
  • Staphylococcus / immunology*
  • Staphylococcus aureus / immunology*
  • Superantigens*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Toxins
  • Calcium-Binding Proteins
  • Enterotoxins
  • Spec1 protein, Strongylocentrotus purpuratus
  • Superantigens
  • enterotoxin F, Staphylococcal
  • enterotoxin B, staphylococcal
  • enterotoxin C, staphylococcal
  • Cysteine Endopeptidases
  • streptopain