Abstract
Brain-specific angiogenesis inhibitor 1 (BAI1) is a p53-target gene specifically expressed in the brain. We examined the distribution of the endogenous BAI1 protein in normal human brain tissue using a polyclonal antibody against the extracellular region of BAI1. Immunohistochemical study demonstrated that BAI1 was expressed in neuronal cells of the cerebral cortex but not in astrocytes. BAI1 protein was localized in the cellular cytoplasm and membrane. It was predominantly localized in the cellular membrane when expressed in cultured cells by means of gene transfection. BAI1 protein may play an important role in neuronal functions such as synapse formation and signal transduction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Angiogenesis Inhibitors
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Angiogenic Proteins*
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Animals
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Astrocytes / cytology
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Astrocytes / metabolism*
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Blotting, Western
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COS Cells
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Cell Compartmentation / physiology
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Cell Membrane / metabolism*
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Cerebral Cortex / cytology
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Cerebral Cortex / metabolism*
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Cytoplasm / metabolism*
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Fluorescent Antibody Technique
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Glial Fibrillary Acidic Protein / metabolism
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Humans
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Male
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Neurites / metabolism
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Neurites / ultrastructure
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Neurons / cytology
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Neurons / metabolism*
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Proteins / genetics
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Proteins / metabolism*
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Receptors, G-Protein-Coupled
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Transfection
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Tumor Suppressor Protein p53 / metabolism*
Substances
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ADGRB1 protein, human
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Angiogenesis Inhibitors
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Angiogenic Proteins
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Glial Fibrillary Acidic Protein
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Proteins
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Receptors, G-Protein-Coupled
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Tumor Suppressor Protein p53