Human aldolase C gene expression is regulated by adenosine 3',5'-cyclic monophosphate (cAMP) in PC12 cells

Gene. 2002 May 29;291(1-2):115-21. doi: 10.1016/s0378-1119(02)00586-3.

Abstract

We have examined the effects of an adenosine 3',5'-cyclic monophosphate (cAMP) analog on human aldolase C gene expression in the rat pheochromocytoma cell line PC12. Incubation for 4 h with 500 microM 8-Br-cAMP increased aldolase C mRNA expression 2.5-fold and the expression was still above basal level 24 h later. Using transient transfection experiments we demonstrate that the distal element D in the promoter region of the human aldolase C gene, which binds a transcriptional activator (NGFI-B), is involved in this regulation. NGFI-B mRNA and protein expression were promptly (15 min) increased after 8-Br-cAMP treatment and precedes aldolase C mRNA increase (30 min). After 4 h of 8-Br-cAMP treatment, the binding of NGFI-B protein to the distal element D in the distal promoter region was increased twofold and this correlates with the increased expression of the clone that contains distal element D. These results indicate that the distal element D in the promoter region of the human aldolase C gene is the target of a cAMP-dependent regulation pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Animals
  • Chloramphenicol O-Acetyltransferase / drug effects
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Cyclic AMP / pharmacology*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fructose-Bisphosphate Aldolase / genetics*
  • Gene Expression / genetics
  • Gene Expression Regulation, Enzymologic / drug effects
  • Humans
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • PC12 Cells
  • Promoter Regions, Genetic / genetics
  • Protein Binding / drug effects
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Response Elements / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Up-Regulation / drug effects

Substances

  • DNA-Binding Proteins
  • NR4A1 protein, human
  • Nr4a1 protein, rat
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Recombinant Fusion Proteins
  • Transcription Factors
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Cyclic AMP
  • Chloramphenicol O-Acetyltransferase
  • Fructose-Bisphosphate Aldolase