In vitro susceptibility to infection with SIVcpz and HIV-1 is lower in chimpanzee than in human peripheral blood mononuclear cells

J Med Virol. 2002 Jul;67(3):301-11. doi: 10.1002/jmv.10078.

Abstract

This study was undertaken to evaluate and compare the susceptibility of chimpanzee versus human peripheral blood mononuclear cells (PBMCs) to infection with SIVcpz and HIV-1 non-syncitium inducing primary isolates. The results demonstrate clearly that chimpanzee PBMCs have a lower capacity to support viral replication as compared to human PBMCs. There was no experimental evidence that this difference was due to a lower availability of target cells for viral infection (PBMCs positive for CD4 and CCR5 molecules) or to a differential susceptibility to apoptosis (PBMCs positive for CD4 and CD95 molecules). A lower capacity of chimpanzee PBMCs to support SIVcpz and HIV-1 replication in vitro is related to a post-entry barrier to virus replication.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Chemokines, CC / metabolism
  • DNA, Viral / blood
  • Disease Susceptibility
  • HIV Infections / virology
  • HIV-1 / pathogenicity*
  • HIV-1 / physiology
  • Humans
  • Immunophenotyping
  • Leukocytes, Mononuclear / virology*
  • Pan troglodytes
  • Proviruses
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / pathogenicity*
  • Simian Immunodeficiency Virus / physiology
  • Species Specificity
  • Viral Load
  • Virus Replication

Substances

  • Chemokines, CC
  • DNA, Viral