Abstract
A PCR-based technique using molecular beacons was developed to detect the chloroquine resistance-associated pfcrt K76T point mutation in Plasmodium falciparum. One hundred thirty African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This rapid and inexpensive genomic assay could expand the possibilities for monitoring chloroquine resistance.
MeSH terms
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Animals
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Antimalarials / pharmacology*
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Chloroquine / pharmacology*
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Cloning, Molecular
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DNA, Protozoan / genetics
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Drug Resistance
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Fluorescent Dyes
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Genetic Markers
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Humans
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Malaria, Falciparum / parasitology*
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Membrane Proteins / genetics
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Membrane Transport Proteins
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Plasmodium falciparum / drug effects*
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Plasmodium falciparum / genetics*
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Point Mutation / genetics
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Protozoan Proteins
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Antimalarials
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DNA, Protozoan
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Fluorescent Dyes
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Genetic Markers
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Membrane Proteins
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Membrane Transport Proteins
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PfCRT protein, Plasmodium falciparum
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Protozoan Proteins
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Chloroquine