Riboflavin is a component of the Na+-pumping NADH-quinone oxidoreductase from Vibrio cholerae

Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10322-4. doi: 10.1073/pnas.162361299. Epub 2002 Jul 16.

Abstract

Flavins are cofactors in many electron-transfer enzymes. Typically, two types of flavins perform this role: 5'-phosphoriboflavin (FMN) and flavin-adenine dinucleotide (FAD). Both of these are riboflavin derivatives, but riboflavin itself has never been reported to be an enzyme-bound component. We now report that tightly bound riboflavin is a component of the NADH-driven sodium pump from Vibrio cholerae.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Flavin-Adenine Dinucleotide
  • Molecular Structure
  • Protein Denaturation
  • Quinone Reductases / analysis*
  • Riboflavin / analogs & derivatives*
  • Riboflavin / analysis*
  • Sodium-Potassium-Exchanging ATPase*
  • Vibrio cholerae / enzymology*

Substances

  • Bacterial Proteins
  • riboflavin 5'-phosphorothioate
  • Flavin-Adenine Dinucleotide
  • sodium-translocating NADH-quinone reductase
  • Quinone Reductases
  • Sodium-Potassium-Exchanging ATPase
  • Riboflavin