Abstract
Rhesus monkey rhadinovirus (RRV) is a gamma2-herpesvirus identified from the peripheral blood mononuclear cells of rhesus macaques (Macaca mulatta). Serologic studies suggested that the infections of RRV are prevalent among rhesus monkeys. RRV can be efficiently propagated and grows to a high titer in cultured rhesus monkey fibroblasts, thus providing a valuable model to study the rhadinovirus replication. By comparative genomic studies, here we describe the identification of two potential transcriptional factors encoded by RRV, designated as R-Rta and R-bZIP. Initial functional characterization of these products suggested that R-Rta is a potent transcriptional activator and R-bZIP forms homodimers in vivo. Viral homologues of R-Rta and R-bZIP, previously identified from other rhadinoviruses, have been implicated in serving as molecular switches in lytic replication.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cells, Cultured
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Genome, Viral
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Heterotrimeric GTP-Binding Proteins / genetics*
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Heterotrimeric GTP-Binding Proteins / metabolism
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Leucine Zippers
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Macaca mulatta / virology*
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Molecular Sequence Data
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RNA, Messenger / analysis
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RNA, Viral / analysis
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Rhadinovirus / chemistry
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Rhadinovirus / genetics*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcriptional Activation
Substances
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DNA-Binding Proteins
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Mrgprf protein, rat
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RNA, Messenger
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RNA, Viral
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Receptors, Cell Surface
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Transcription Factors
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Heterotrimeric GTP-Binding Proteins
Associated data
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GENBANK/AF241163
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GENBANK/AF241164