Abstract
Targeting CD22 on human B-cells with a monoclonal antibody conjugated to a cytotoxic RNAse causes potent and specific killing of the lymphoma cells in vitro. This translates to anti-tumor effects in human lymphoma models in SCID mice. RNA damage caused by RNAses could be an important alternative to standard DNA-damaging chemotherapeutics. A second generation construct with an improved recombinant cytotoxic RNAse is described. Targeted RNAses may overcome problems of toxicity and immunogenicity associated with plant or bacterial toxin-containing immunoconjugates.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Antibodies, Monoclonal / therapeutic use
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Antigens, CD
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Antigens, Differentiation, B-Lymphocyte
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Cell Adhesion Molecules*
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Humans
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Immunotoxins / therapeutic use*
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Lectins / antagonists & inhibitors*
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Lymphoma, B-Cell / drug therapy*
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Mice
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Mice, SCID
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Ribonucleases / therapeutic use*
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Sialic Acid Binding Ig-like Lectin 2
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation, B-Lymphocyte
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CD22 protein, human
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Cd22 protein, mouse
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Cell Adhesion Molecules
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Immunotoxins
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Lectins
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Sialic Acid Binding Ig-like Lectin 2
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Ribonucleases
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rapLR1 enzyme