Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells

J Neuroimmunol. 2002 Aug;129(1-2):66-73. doi: 10.1016/s0165-5728(02)00178-9.

Abstract

Experiments were conducted in both HEK cells and cerebellar neurons to investigate whether CXC chemokine receptor 2 (CXCR2) is functionally coupled to GluR1. The co-expression of CXCR2 with GluR1 in HEK cells increased (i) the GluR1 "apparent" affinity for the transmitter; (ii) the GluR1 channel open probability; and (iii) GluR1 binding site cooperativity upon CXCR2 stimulation with CXC chemokine ligand 2 (CXCL2). The affinity of C-terminal-deleted GluR1 for glutamate (Glu) remained stable instead. Furthermore, CXCL2 increased the binding site cooperativity of AMPA receptors in rat cerebellar granule cells; and the amplitude of spontaneous excitatory postsynaptic current (sEPSCs) in Purkinje neurons (PNs). Our findings indicate that the coupling of CXCR2 with GluR1 may modulate glutamatergic synaptic transmission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites / drug effects
  • Binding Sites / immunology
  • Cells, Cultured
  • Central Nervous System / immunology
  • Central Nervous System / metabolism*
  • Cerebellar Cortex / drug effects
  • Cerebellar Cortex / immunology
  • Cerebellar Cortex / metabolism
  • Chemokines, CXC / immunology
  • Chemokines, CXC / metabolism*
  • Chemokines, CXC / pharmacology
  • DNA, Complementary / genetics
  • Dose-Response Relationship, Drug
  • Excitatory Amino Acid Antagonists / pharmacology
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / immunology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / immunology
  • Glutamic Acid / metabolism*
  • Glutamic Acid / pharmacology
  • Humans
  • Ion Channels / genetics
  • Ion Channels / immunology
  • Neurons / drug effects
  • Neurons / immunology
  • Neurons / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar
  • Receptors, AMPA / genetics
  • Receptors, AMPA / immunology
  • Receptors, AMPA / metabolism*
  • Receptors, Interleukin-8B / genetics
  • Receptors, Interleukin-8B / immunology
  • Receptors, Interleukin-8B / metabolism*
  • Synapses / immunology
  • Synapses / metabolism*
  • Synaptic Transmission / immunology*

Substances

  • Chemokines, CXC
  • DNA, Complementary
  • Excitatory Amino Acid Antagonists
  • Ion Channels
  • Receptors, AMPA
  • Receptors, Interleukin-8B
  • Glutamic Acid
  • glutamate receptor ionotropic, AMPA 1

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