Abstract
Stress has a negative impact on wound healing. This murine study evaluated the effect of restraint stress (RST) on interleukin-1 (IL-1) and keratinocyte growth factor-1 (KGF-1) gene expression in cutaneous wounds by in situ hybridization. At day 1, RST mice had reduced frequency of IL-1beta mRNA-expressing fibroblasts compared to control mice. At day 3, RST mice had reduced frequencies of IL-1beta mRNA-expressing leukocytes, KGF-1 mRNA-expressing fibroblasts, and thin proliferating zones of epithelium. At day 5, RST mice had persistent leukocytic foci and reduced granulation tissue. Therefore, stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Down-Regulation / genetics
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Down-Regulation / immunology
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Female
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Fibroblast Growth Factor 7
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Fibroblast Growth Factors / genetics*
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Fibroblasts / immunology*
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Gene Expression Regulation / immunology
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In Situ Hybridization
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Inflammation / genetics*
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Inflammation / immunology
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Inflammation / physiopathology
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Interleukin-1 / genetics*
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Mice
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Mice, Inbred Strains
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RNA, Messenger / immunology
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RNA, Messenger / metabolism
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Restraint, Physical
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Skin / immunology*
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Skin / injuries
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Skin / physiopathology
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Stress, Physiological / genetics*
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Stress, Physiological / immunology
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Stress, Physiological / physiopathology
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Wound Healing / genetics*
Substances
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Fgf7 protein, mouse
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Interleukin-1
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RNA, Messenger
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Fibroblast Growth Factor 7
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Fibroblast Growth Factors