Abstract
Fourteen different chimeric virus genomes were constructed from two infectious cDNA clones encoding a virulent and an attenuated isolate, respectively, of the HM175 strain of hepatitis A virus. The ability of each recombinant virus to infect tamarins and to cause acute hepatitis was determined. Comparisons of the genotype and phenotype of each virus suggested that VP1/2A and 2C genes were responsible for virulence. The 2C gene derived from the attenuated parent virus was unstable, and one or more mutations arose in this gene during the first passage in tamarins.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cysteine Endopeptidases / genetics*
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Cysteine Endopeptidases / metabolism
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Genotype
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Hepatitis A / physiopathology
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Hepatitis A / virology
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Hepatitis A virus / genetics
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Hepatitis A virus / pathogenicity*
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Mutation*
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Phenotype
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Recombination, Genetic
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Saguinus
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Viral Nonstructural Proteins / genetics*
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Viral Nonstructural Proteins / metabolism
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Viral Proteins*
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Viral Structural Proteins / genetics*
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Viral Structural Proteins / metabolism
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Virulence / genetics
Substances
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Carrier Proteins
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VP1 protein, hepatitis A virus
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Viral Nonstructural Proteins
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Viral Proteins
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Viral Structural Proteins
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Cysteine Endopeptidases
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picornain 2A, Picornavirus
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2C protein, viral