Cosine modulated HSQC: a rapid determination of (3)J(HNHalpha) scalar couplings in (15)N-labeled proteins

J Magn Reson. 2002 Jun;156(2):313-7. doi: 10.1006/jmre.2002.2538.

Abstract

A two-dimensional HSQC-based NMR method, (15)N-COSMO-HSQC, is presented for the rapid determination of homonuclear (3)J(HNHalpha) couplings in (15)N-labeled proteins in solution. Scalar couplings are extracted by comparing the intensity of two separate datasets recorded with and without decoupling of the (3)J(HNHalpha) during a preparation period. The scalar couplings are introduced through a cosine modulation of the peak intensities. The experiment relies on a BIRD sandwich to selectively invert all amide protons H(N) and is very simple to implement. (3)J(HNHalpha) couplings were determined using both the (15)N-COSMO-HSQC and quantitative-J on (15)N-labeled chemokine RANTES. The two experiments show well-correlated values.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mathematics
  • Nuclear Magnetic Resonance, Biomolecular*
  • Proteins / chemistry*

Substances

  • Proteins