Tandem satellite DNA repeats are often associated with centromeres. In spite of their importance in the organization of the centromere, they do not seem to be broadly conserved among species and their role is still unclear. Here we report the identification of a new 44-bp tandem pericentromeric repeat from the medfly, Ceratitis capitata. The repeat is specific to this insect and is not found in any of the other closely related species tested. It localizes in four out of its five autosomes and in the X chromosome. It is organized in long arrays, interspersed by transposable elements and other less well-defined sequence motifs.