Copper homeostasis and aging in the fungal model system Podospora anserina: differential expression of PaCtr3 encoding a copper transporter

Int J Biochem Cell Biol. 2002 Nov;34(11):1355-71. doi: 10.1016/s1357-2725(02)00078-x.

Abstract

Lifespan extension of Podospora anserina mutant grisea is caused by a loss-of-function mutation in the nuclear gene Grisea. This gene encodes the copper regulated transcription factor GRISEA recently shown to be involved in the expression of PaSod2 encoding the mitochondrial manganese superoxide dismutase. Here we report the identification and characterization of a second target gene. This gene, PaCtr3, encodes a functional homologue of the Saccharomyces cerevisiae high affinity copper permease yCTR3. PaCtr3 is not expressed in the grisea mutant confirming the assumption that the extension of lifespan is primarily caused by cellular copper limitation and a switch from a cytochrome oxidase (COX)-dependent to and alternative oxidase (AOX)-dependent respiration. Transcript levels of PaCtr3 and PaSod2 respond to copper, iron, manganese and zinc. Transcription of PaCtr3 was found to be down-regulated during senescence of wild-type cultures suggesting that the intracellular copper concentration is raised in old cultures. A two hybrid analysis suggested that GRISEA acts as a homodimer. In accordance, an inverted repeat was identified as a putative binding sequence in the promoter region of PaCtr3 and of PaSod2. Finally, the expression of PaCtr3 in transformants of the grisea mutant led to lifespan shortening. This effect correlates with the activity of the copper-dependent COX demonstrating a strong link between copper-uptake, respiration and lifespan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Amino Acid Sequence
  • Antiporters / chemistry
  • Antiporters / genetics
  • Antiporters / metabolism*
  • Cation Transport Proteins
  • Copper / metabolism*
  • Dimerization
  • Fungal Proteins*
  • Genes, Fungal
  • Homeostasis*
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism*
  • Molecular Sequence Data
  • Phenotype
  • Promoter Regions, Genetic
  • SLC31 Proteins
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sordariales / genetics
  • Sordariales / metabolism*
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Two-Hybrid System Techniques

Substances

  • Antiporters
  • CTR3 protein, S cerevisiae
  • Cation Transport Proteins
  • Fungal Proteins
  • GRISEA protein, Podospora anserina
  • Membrane Transport Proteins
  • SLC31 Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Copper
  • Superoxide Dismutase