IgE to Bet v 1 and profilin: cross-reactivity patterns and clinical relevance

J Allergy Clin Immunol. 2002 Sep;110(3):435-42. doi: 10.1067/mai.2002.126380.

Abstract

Background: Individuals with pollen allergy often have IgE against plant-derived foods. This can be due to cross-reactive IgE against Bet v 1 and homologues, profilins, and/or cross-reactive carbohydrate determinants.

Objective: The aim of this study was to correlate sensitization to Bet v 1 and profilin with individual recognition patterns to plant foods and clinical relevance.

Methods: Fifty-two patients with pollen allergy and IgE against at least one plant-derived food were included in the study. Adverse reactions to plant-derived foods were documented by using standardized interviews. Skin prick tests were performed for pollen (grass, birch, and mugwort) and 14 plant-derived foods. In addition, recombinant (r) Bet v 1 and rBet v 2 (profilin) were tested intracutaneously. Specific IgE against the abovementioned allergens were determined by means of RAST. Cross-reactivity was studied by means of RAST inhibition.

Results: Eighty-five percent of patients were sensitized to Bet v 1, and 71% were sensitized to profilin. Profilin was associated with a higher number of positive RAST results to plant-derived foods than Bet v 1. In contrast, Bet v 1 was associated with more positive skin prick test responses and more food-related symptoms. Sensitization to Bet v 1 was associated with IgE against apple, hazelnut, and peach, whereas sensitization to profilin was associated with positive RAST results to all investigated plant-derived foods except apple, peach, and melon.

Conclusions: IgE antibodies against Bet v 1 have a more limited spectrum of cross-reactivity than those against profilin, but they frequently give rise to clinically relevant cross-reactivities to food. In analogy to anticarbohydrate IgE, cross-reactive IgE against food profilins have no or very limited clinical relevance.

MeSH terms

  • Adolescent
  • Adult
  • Allergens / immunology
  • Antigens, Plant
  • Contractile Proteins*
  • Cross Reactions
  • Female
  • Food Hypersensitivity / diagnosis
  • Food Hypersensitivity / immunology
  • Humans
  • Hypersensitivity, Immediate / classification
  • Hypersensitivity, Immediate / diagnosis*
  • Hypersensitivity, Immediate / immunology*
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology*
  • Male
  • Microfilament Proteins / immunology*
  • Middle Aged
  • Plant Proteins / immunology*
  • Profilins
  • Radioallergosorbent Test
  • Rhinitis, Allergic, Seasonal / diagnosis
  • Rhinitis, Allergic, Seasonal / immunology

Substances

  • Allergens
  • Antigens, Plant
  • Contractile Proteins
  • Microfilament Proteins
  • Plant Proteins
  • Profilins
  • Bet v 1 allergen, Betula
  • Immunoglobulin E