BXD recombinant inbred mice represent a novel T cell-mediated immune response tumor model

Int J Cancer. 2002 Sep 20;101(3):270-9. doi: 10.1002/ijc.10606.

Abstract

To develop a better animal model for studying the effects of the host environment in neoplasia, we injected various genetically well-characterized H-2(d) RI strains of BXD mice with syngeneic breast cancer cells (TS/A) and monitored the growth of tumors over time. There was a marked difference in the growth of the implanted breast cancer cells among the 14 BXD RI strains, with 4 patterns of tumor development being observed: in type I, the implanted tumor cells grew rapidly in the first 2 weeks, necrosis of the tumors was observed and metastases to the intestinal lymph nodes and pancreas was observed, causing death; in type II, the implanted tumor cells grew slowly and attained a size after day 50 that required killing the animal, with tumor necrosis being rare and metastases absent; in type III, the implanted tumor cells grew initially but underwent a slow decline after 4 weeks; and in type IV, the implanted tumor cells failed to develop. Apoptosis of the implanted tumor cells was responsible for the regression of tumor nodules. The T-cell immune response minimized tumor development in types III and IV since T-cell depletion of the BXD RI mice resulted in aggressively growing tumors in these strains.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Breast Neoplasms / immunology*
  • Breast Neoplasms / pathology
  • Disease Models, Animal
  • Female
  • Genetic Predisposition to Disease
  • Immunity, Cellular
  • Immunophenotyping
  • Lymph Nodes / immunology
  • Major Histocompatibility Complex / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Neoplasm Transplantation / immunology
  • Neoplasm Transplantation / pathology
  • T-Lymphocytes / immunology*
  • Transplantation, Isogeneic
  • Tumor Cells, Cultured