[Determination of flavonoids in pollen typhae(Puhuang) by HPCE and HPLC]

Zhongguo Zhong Yao Za Zhi. 1999 Nov;24(11):682-4, 703.
[Article in Chinese]

Abstract

Objective: To determine two flavonoids isorhamnetin-3-O-neohesperidoside(I) and typheneoside(II) in Pollen Typhae by HPCE and HPLC.

Method: HPCE conditions: buffer = 0.02 mol.L-1 borate, 0.05 mol.L-1 SDS and 10% acetonitrile; V = 20 kV; T = 30 degrees C; lambda = 270 nm; sample size = 30 kPa.s, HPLC conditions: C18 lumn, acetonitrile-water (15:85) as mobile phase detection wavelength at 254 nm.

Result: The contents of flavonoids I and II in five different Typha species and eighteen commercial samples of traditional Chinese drug Puhuang were between 0.1%-0.6%.

Conclusion: These two methods are rapid, simple, accurate and can be used for the quality control of this traditional Chinese drug.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Electrophoresis, Capillary
  • Flavonols
  • Glycosides / analysis*
  • Hesperidin / analogs & derivatives
  • Hesperidin / analysis*
  • Magnoliopsida / chemistry*
  • Magnoliopsida / classification
  • Pollen / chemistry
  • Quercetin / analogs & derivatives
  • Quercetin / analysis*

Substances

  • Flavonols
  • Glycosides
  • isorhamnetin-3-O-neohesperidoside
  • typhaneoside
  • Quercetin
  • Hesperidin