Objective: To screen HCV NS5 mimotopes by using monoclonal antibody and phage peptide library.
Methods: By using HCV NS5 monoclonal antibody as selective molecule, a 7 peptide phage library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
Results: Twelve positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as the mimotope of HCV NS5.
Conclusions: HCV mimotope is obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.