-11 Mutation in the ampC promoter increasing resistance to beta-lactams in a clinical Escherichia coli strain

S Corvec; N Caroff; E Espaze; J Marraillac; A Reynaud

doi:10.1128/AAC.46.10.3265-3267.2002

-11 Mutation in the ampC promoter increasing resistance to beta-lactams in a clinical Escherichia coli strain

Antimicrob Agents Chemother. 2002 Oct;46(10):3265-7. doi: 10.1128/AAC.46.10.3265-3267.2002.

Authors

S Corvec¹, N Caroff, E Espaze, J Marraillac, A Reynaud

Affiliation

¹ Laboratoire de Bactériologie-Virologie, Hygiène hospitalière, CHU, Université de Nantes, France. [email protected]

Abstract

A mutation was discovered in the Pribnow box of the ampC promoter in a clinical Escherichia coli strain. This -11 C-to-T transition created a perfect homology with the -10 consensus sequence. The new promoter was cloned upstream of the cat gene of pKK232-8 and induced a sixfold increase in promoter strength.

MeSH terms

Bacterial Proteins*
Base Sequence
Escherichia coli / drug effects*
Escherichia coli / enzymology
Escherichia coli / genetics
Humans
Microbial Sensitivity Tests
Mutation*
Promoter Regions, Genetic*
beta-Lactam Resistance*
beta-Lactamases / genetics*
beta-Lactams / pharmacology

Substances

Bacterial Proteins
beta-Lactams
AmpC beta-lactamases
beta-Lactamases