Abstract
Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its interactions with these two proteins, Mlc1p plays a role in polarized growth and cytokinesis. Mlc1p has been crystallized in complexes with four different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4 and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple anomalous dispersion (MAD) experiments based on selenomethionine derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Binding Sites
-
Cell Division
-
Cloning, Molecular
-
Escherichia coli
-
Models, Molecular
-
Molecular Sequence Data
-
Myosin Light Chains / chemistry*
-
Myosin Light Chains / isolation & purification
-
Myosin Light Chains / metabolism
-
Myosin Type V / chemistry*
-
Myosin Type V / metabolism
-
Protein Conformation
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / isolation & purification
-
Recombinant Proteins / metabolism
-
Saccharomyces cerevisiae / growth & development
-
Saccharomyces cerevisiae / physiology
-
Saccharomyces cerevisiae Proteins / chemistry
-
Saccharomyces cerevisiae Proteins / isolation & purification
-
Saccharomyces cerevisiae Proteins / metabolism
-
X-Ray Diffraction / methods
Substances
-
MLC1 protein, S cerevisiae
-
Myosin Light Chains
-
Recombinant Proteins
-
Saccharomyces cerevisiae Proteins
-
Myosin Type V